These spore prints are created by allowing mature mushrooms to release their spores onto a sterile petri dish. These spores are the reproductive units of the mushroom, similar to seeds in plants.
Once the spore print is collected, it can be used to cultivate new mushroom colonies. Spore prints contain a multitude of spores, each potentially capable of growing into a new mushroom organism. Cultivators use these spores to inoculate a suitable growing medium, such as a substrate composed of nutrient-rich materials. Under controlled conditions, the spores germinate, develop into mycelium (the vegetative part of the fungus), and eventually form the mushroom's fruiting body.